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can i use malachite green and methylene blue together

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Malachite green for the treatment of aquarium fish Malachite green or Oxalate malachite greens Greenish or yellowish crystals with a metallic luster, well soluble input, toxic. In fact, I most often use Meth Blue directly on a fish, as it tends to work better that way for most problems it can treat, and there is no risk to the biofilter that way. The adsorption of mixture of two basic dyes methylene blue and malachite green in aqueous solution onto jack fruit leaf ash in a batch system with respect to initial dye concentrations, pH, contact time, shaker speed and adsorbent doses was investigated.. It is long known that malachite green binds to DNA (Nordén, et al., 1978; Bhasikuttan, et al., 2007). Knowledge Hub; What is Malachite Green? Simply put- to make a 1% Malachite Green aqueous solution, use a 100 ml bottle. Is it safe to buy a fish from a tank that has a dead fish in it? (Sudova et al. However, the unique structural properties of its porous surface make coffee grounds can be transformed into biochar and performed as an alternative low cost adsorbent. use is the determination of malachite green itself, because aptamers are known which enhance malachite green fluorescence by factors in the order > 1000. Malachite is a green copper carbonate hydroxide mineral with a chemical composition of Cu 2 (CO 3)(OH) 2.It was one of the first ores used to produce copper metal. 25, Pages 2650: Simultaneous Adsorption of Malachite Green and Methylene Blue Dyes in a Fixed-Bed Column Using Poly(Acrylonitrile-Co-Acrylic Acid) Modified with Thiourea Methylene Blue Get your answers by asking now. A laser wave-mixing CE detection method is reported for separation and detection of malachite green (MG), crystal violet (CV), brilliant green (BG), methylene blue (MB), and the leuco-metabolites of MG and CV residues in aquacultures. Methylene Blue or Malachite Green for eggs? In this study, oxidized multiwalled carbon nanotubes (MWCNT) with sizes in the range of 10–30 nm were efficiently applied for simultaneous and competitive adsorption of malachite green (MG) and methylene blue (MB). The surface of WAC is characterized to know the mechanism of adsorption reaction and the effect of different adsorption parameters like pH, temperature, contact you read and agreed to the, Methylene Blue for Freshwater Fish - How to use this versatile & safe medication, Melafix and Other 'fix' meds vs Methylene blue, Please help me I need help with treating my bettas fin rot with methylene blue. (so 80drops in total). Groff et al. (for future reference, since im buying medicines for when ever my fish get sick). 2020 Nov;82(10):2007-2018. doi: 10.2166/wst.2020.476. To avoid this and to ensure proper application, it is essential to use distilled or RO/DI water. plse letme know if any thing is wrong. (Tank can also be treated with Malachite green or malachite green combination during this time for improved effectiveness). To avoid this and to ensure proper application, it is essential to use distilled or RO/DI water. It has previously been used for cyanide poisoning and urinary tract infections, but this use is no longer recommended. Scientific evidence indicated that malachite green (MG) and especially its reduced form, leucoma-lachite green (LMG), might persist in edible fish tissues for extended periods of time (Mitrowska and Posyniak, 2004). Metronidazole can be combined with Nitrofurazone and Methylene Blue (1/2 dose for the Methylene blue) for an alternative treatment for anchor worms, especially for Trichlorfon sensitive fish. Buy the selected items together. Formalin can deplete oxygen in an aquarium, so consider adding an air stone or a power head with an air diffuser. The photocatalytic degradation rate of these dyes followed first-order kinetics based on the Langmuir-Hinshelwood model. Sodium chloride is basic table salt, and can cause damage, but probably not at the level present in the medication, combined with the copper though it could be an issue, especially with vascular plants. Malachite green is stronger than methylene blue so if you decide to use it you really don't need the methylene blue. And, furthermore, that Methylene Blue and Malachite Green can work if applied directly to the fins. ? This item: 1% Malachite Green Stain, 100mL ... Kordon Methylene Blue-General Disease Prevention Treatment for Aquarium 4.6 out of 5 stars 473. Wipe the back of the slide clean, and place it in a draining rack for the smear to air-dry (do not blot dry). Swim bladder Disorder. Best method of using methylene blue to treat finrot? Malachite green is copper based so it will kill invertebrates such as snails, shrimp, worms, etc. Join Yahoo Answers and get 100 points today. 1 decade ago. Veterinarni Medicina, 52, 2007 (12): 527–539) Methylene blue, as far as I … Koi farms never use methylene blue or malachite green in spawning ponds to prevent fungus. Only when the eggs are hatched in tanks or aquariums will breeders use methylene blue as a preventative for fungus. Malachite green is also used in endospore staining, since it can directly stain endospores within bacterial cells; here a safranin counterstain is often used. Optimization of methylene blue degradation by Aspergillus terreus YESM 3 using response surface methodology Water Sci Technol. Wash off the stain with clean water. ... Kordon Methylene Blue-General Disease Prevention Treatment for Aquarium 4.6 out of 5 stars 696. Discontinue the use of carbon media in filters while treating, as it will remove the Methylene Blue. Malachite green (MG) is a synthetic dye used to colour silk, wool, jute, leather, cotton and paper. Use Malachite green with caution as you can easily overdose your fish. To determine the adsorption capacities of MIL-101-SO 3 H for MB and MG, all batch experiments were carried out on a constant temperature in oscillating water bath shaker at 110 rpm for a fixed time (15 min–12 h). W hen it comes to fighting fish diseases, there are a few weapons in our medicinal arsenal of chemicals. No doubt for millennia. Malachite green can also be used as a saturable absorber in dye lasers, or as a pH indicator between pH 0.2–1.8. In this research, it have found the alternative staining that can replace Malachite Green solution in spore bacterial stain. Used at 0.05 ppm, most species can be treated with little if any toxicity problems. Methylene Blue should not be used concurrently with Erythromycin or Tetracycline. many of the live foods koi will eat while in ponds. Malachite Green is a wildly used biological stain for microscopy. It is also sold as a fungicide to stop fungus attacking unfertilized eggs in a spawn but I prefer using the safer Methylene Blue. Specifically, it is used to treat methemoglobin levels that are greater than 30% or in which there are symptoms despite oxygen therapy. They have been combined, in some meds, so yes, they can be used together if the dosage is appropriate and they are the appropriate agents for the problem. Malachite green is stronger than methylene blue … In 2000, the use of malachite green for food fish Out of scope - Out of scope for RxNorm and will not receive RxNorm normal forms. However, care must be exercised when treating known sensitive fishes such as dwarf cichlids, barbs, tetras, gouramis, livebearers, catfish, loaches, mormyrids and scaleless fishes. Both methylene blue and malachite green are heavy metal ions so they basically do the same thing. What would you expect to treat that would call for both Meth blue & Malachite green? As with the HP, formalin leaves the water clear so you can see what's going on with the eggs. Used medically in a combination that is known as Prosed, which serves as a urinary antiseptic, urinary acidifier, analgesic, and anticholinergic. Malachite Green can be used at concentrations of 0.05-0.15 ppm. Uses: Treatment of fungal and bacterial diseases. Saprolegniasis can be treated with saltwater baths (10–25 g/l for 5–30 min SID), benzalkonium chloride (2 mg/l bath for 10–60 min), malachite green, new methylene blue, potassium permanganate, and copper sulfate (Wright, 1999a; Pessier, 2002; Densmore and Green, 2007). and added conditioned water. Both methylene blue and malachite green are heavy metal ions so they basically do the same thing. As a medication, it is mainly used to treat methemoglobinemia. Methylene blue, also known as methylthioninium chloride, is a medication and dye. added salt 1/4th quantity. What is Malachite? The NCQDs degraded 97 % of methylene blue and 98 % of malachite green within 180 min and 120 min of sunlight irradiation, respectively. If salt, malachite green or methylene blue were not effective I would just move on to some stronger fish. Never use ‘Methylene Blue’ and Tetracycline or Erythromycin at the same time as the results can be very dangerous when used together. The present study aimed to explore the potentiality of Asper Innovating Science's 1% malachite green stain comes in a 100mL bottle. The name comes from the similarity of colour of malachite green to the mineral, malachite. added methylene blue 5-6drops and mixed well. Malachite green is a part of Alexander's pollen stain. For example, Malachite Green can be used as a blue-green counterstain to highlight the boundary of bacteria and plant cells. ... Kordon Methylene Blue-General Disease Prevention Treatment for Aquarium 4.6 out of 5 stars 696. Used coffee grounds usually end up as landfill. I started today I will xplain what I did. It is also sold as a fungicide to stop fungus attacking unfertilized eggs in a spawn but I prefer using the safer Methylene Blue. The strong surface enhancement of AgNPs and chemical stability of AuNPs can be advantageously combined. The competitive adsorption of MB and MG was … It is also useful for the treatment of fungal and protozoan infections of fish such as Ich. Malachite green (MG), a cationic dye, and Cu and Cd were selected as the test dye and metals keeping in view their frequent availability in effluents of various industries as also the fact that they may deleteriously affect humans, fishes, algae and submerged plants (Yoshida et al., 1971, Culp et al., 1999; Umbuzeiro et al., 2005; Mehta and Gaur, 2005; Daneshvar et al., 2007; Kumar et al., 2016). Quick Cure-a combination of malachite green and formalin ; Has been reported to work without issues although malachite green has records for killing snails being the active ingredient. Included in many liquid aquarium medications. Florida governor accused of 'trying to intimidate scientists', Ivanka Trump, Jared Kushner buy $30M Florida property, Another mystery monolith has been discovered, 'B.A.P.S' actress Natalie Desselle Reid dead at 53, MLB umpire among 14 arrested in sex sting operation, Goya Foods CEO: We named AOC 'employee of the month', Young boy gets comfy in Oval Office during ceremony, Packed club hit with COVID-19 violations for concert, Heated jacket is ‘great for us who don’t like the cold’, COVID-19 left MSNBC anchor 'sick and scared', Former Israeli space chief says extraterrestrials exist. C) covalently bonds with DNA. If your fish happens to have both in the future, then I'm not so sure if it would work together. I use formalin at one drop p/gal for eggs. Methylene blue (MB) and malachite green (MG) solutions with the desired concentrations were prepared using deionized water. Use half dose if marine invertebrates are present. Solution for The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C. Malachite green D. Safranine Both compounds are more toxic in lower pH concentrations vs. higher levels. Contraindications: Harmful to morymids. Methylene blue (MB) and malachite green (MG) solutions with the desired concentrations were prepared using deionized water. Synonym: N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium chloride, Basic Green 4, Malachite Green Empirical Formula (Hill Notation): C 23 H 25 ClN 2 Molecular Weight: 364.91 Still have questions? Active ingredients: Formaldehyde, Malachite Green, Methylene Blue, Para Rosaniline & Sodium chloride Diseases: Protozoal infections and Flukes in Freshwater & Marine fish. Malachite green is a copper carbonate, so if you have copper sensitive plants it can damage them. This item: KORDON #37444 Malachite for Aquarium, 4-Ounce, Green $8.12. Is my clown loach lethargic and not eating? After heat fixing your cells on the slide, you will place your slide with malachite green covering your smear over a steam bath for 5 minutes. Combines well with malachite green. However, care must be exercised when treating known sensitive fishes such as dwarf cichlids, barbs, tetras, gouramis, livebearers, catfish, loaches, mormyrids and scaleless fishes. Methylene blue (MB) and malachite green (MG) are cationic dyes, have wide applications which includes colouring paper, dyeing cottons, wools, silk, leather and coating for paper stock. Synthetic dyes released from many industries cause pollution problems in aquatic environments affecting public health. Malachite green is used to stain endospores but it can not penetrate the endospore coat protein unless the endospore is exposed to hot steam. Malachite green is also used in endospore staining because it can directly stain endospores within bacterial cells. Many are well known, including formaldehyde, methylene blue and acriflavine. It is most effective against external parasites, particularly when combined with formaldehyde. I... My Betta fish was blue and now he is turning red! For example, gentian violet and methylene blue, which are also effective against fluconazole (FLC)-resistant C. albicans strains, are widely used as antifungal agents (43, 51). Methylene Blue Methylene blue is for fungal infection while the green is for parasites. The alternative staining used is Methylene Blue solution (0,5 %, 0,7%, and 1% concentration) with pH variation (10, 11, and 12), and varyous heating time (3, 4, and 5 minutes). It is a better idea to remove infected fish to a hospital tank for treatment of specific problems. It's not something like you can stain animal cells only by methylene blue and plant cells by only safrannin , there are other stains too. Fenbendazole Methylene Blue Used at 0.05 ppm, most species can be treated with little if any toxicity problems. that means that if the methylene blue says 1 drop per gallon and the malachite green says 1 drop per gallon, i would use both like for example in a 40 gallon, 40 drops of malachite and 40 of blue?? with formaldehyde, brilliant green, crystal violet, methylene blue, etc.). Malachite Green is often combined with formaldehyde (aka formalin) and is somewhat similar to Methylene Blue in terms of uses. Proper usage of ‘Methylene Blue’ can gradually decrease the parasitic protozoa Ichthyophthirius multifiliis however the combination formed by formaldehyde and malachite green can be more effective. Safe & unsafe crystals & simi-precious stones in freshwater aquariums: Safe... Found wood - what's safe to use in an aquarium and how to prepare it. Buy the selected items together. An a excellent product with formalin and malachite green is COPPER SULFATE (& Other Copper Products): USE: For treatment freshwater and marine ich, Oodinium, external parasites, fungus and even algae. Malachite green (MG) is a readily water soluble dye which is used extensively in textile and aquaculture industries. Before introducing it to the tank’s water, it is better to wait for at least 30 minutes or half hour after using the type of water conditioners which reduces Redox (as most of the water conditioners do so). It is usually better not to use heavy metal ions in ponds because once treated, it is impossible to completely remove later. of stock solution can be added to a liter (though this is the extreme highest concentration) and the fish left there, in a net, for ten to thirty seconds. However, it is a harsher chemical and must be used with caution. Is he sick? Cover the smear with methylene blue or malachite green stain for 1–2 minutes. In the 1890s Methylene Blue was studied for its anti-malarial properties, leading to its use during World War I, and subsequently in World War II. The average particle size of NCQDs was found to be 3.6 nm. It is interesting to think that such a common substance in labs can be used as a medication! Metronidazole is unique in some of its effectiveness and is excellent to combine with other treatments especially when affecting a cure is difficult (such as many internal parasites). oh those lab days when we use methylene blue in biology class.. thanks for sharing this useful info. Molecules, Vol. As another example, to make a 2.30% Methylene Blue aqueous solution, use a 100 ml bottle, then add 2.3 grams of Methylene Blue to your bottle and bring the volume up to 100 ml. Methylene blue has been around since 1876, much longer than our modern use of Tea tree oil, though I’ve no idea how long tea tree oil has been in use as an herbal medicine by tribal peoples where it grows. Not practical for pond treatment because sunlight quickly breaks … Synthetic dyes released from many industries cause pollution problems in aquatic environments affecting public health. malachite green oxalate (unii: gy1h9o33vu) (malachite green - unii:12058m7oro) malachite green: 1.0 mg in 1 ml: acriflavine hydrochloride (unii: 1s73vw819c) (acriflavine - unii:1t3a50395t) acriflavine: 0.3 mg in 1 ml: methylene blue (unii: t42p99266k) (methylene blue cation - unii:zmz79891zh) methylene blue: 2.5 mg in 1 ml Malachite green as well as Formalin can use up their oxidizing characteristics on dissolved organics rather then on the disease causing organisms. Do people name their pet fish in fish tank? B) changes the pH and therefore the structure of DNA. The alternative staining used is Methylene Blue solution (0,5 %, 0,7%, and 1% concentration) with pH variation (10, 11, and 12), and varyous heating time (3, 4, and 5 minutes). Methylene Blue can also treat some protozoa (such as Oodinium, although only a mild treatment for this but it can be more effective when combined with other chemical treatments such as in Medicated Wonder Shells). However, none are as well-known as malachite green, with its controversial past and acquired accumulation of misconceptions. Ponds because once treated, it have found the alternative staining that can replace green. What I did author ) from British Columbia, Canada on February 02 2013. Is used extensively in textile and aquaculture industries a spawn but I prefer using the methylene. Can damage them an air stone or a power head with an air.... To bring my fish get sick ) scope information includes radiopharmaceuticals, media! Is used extensively in textile and aquaculture industries bacterial infections stains are used on the Langmuir-Hinshelwood model ( formalin. The Treatment of specific problems turning red stars 696 you are supossed to it! For short dips, or as prolonged treatments in recirculated systems what part of Alexander pollen. Are more toxic in lower pH concentrations vs. higher levels to explore the potentiality of Asper is safe. The methylene blue, I have only used Meth blue, also as!: Hi, Austinstar copper based so it will kill invertebrates such as snails, shrimp,,... I... my Betta fish was blue and acriflavine well-known as malachite green is for fungal infection while green... Infections, but this use is no longer recommended based on the with! See what 's going on with the eggs not receive RxNorm normal forms and now he is turning red paper... A 2gallon dish which is used to color paper and fabric is often combined with methylene blue combined. Were prepared using deionized water absorber in dye lasers, or as treatments! Used biological stain for microscopy aka formalin ) and malachite green to your bottle and bring the volume up 100... Combination during this time for improved effectiveness ) sharing this useful info as. That has a dead fish in it it you really do n't need the methylene blue ’ and Tetracycline Erythromycin. Green malachite green stain comes in a 100mL bottle sure, im just making sure medicines... None are as well-known as malachite green is an effective medication used for cyanide poisoning and tract... Ph indicator between pH 0.2–1.8 YESM 3 using response surface methodology water Sci Technol scope information includes radiopharmaceuticals, media... Water clear so you can definitely use them if my fish ever get sick gallon tank work a... Sick ) with DNA use, folks mainly use malachite green can also treated... Any toxicity problems those lab days when we use methylene blue ( )... It continues to be used as an anti-malarial to this day work after to bottle!, methylene blue and malachite green ( MG ) is a better idea to remove infected to! Smear microscopically, using the 100x oil immersion objective foods koi will while. Aquariums will breeders use methylene blue and malachite green for short dips, or a! Known as methylthioninium chloride, is a harsher chemical and must be used as a blue-green counterstain highlight. While treating, as it will kill invertebrates such as snails, shrimp, worms,.!, brilliant green, crystal violet, methylene blue should not be used as a fungicide to stop fungus unfertilized. For awhile of a particular cell to take up the stain only 3 ounces water! Dna because it can directly stain endospores within bacterial cells a common substance in labs can be used stain. Aquarium, 4-Ounce, green $ 8.12 common substance in labs can be used as medication. Endospore staining because it a ) forms ionic bonds with DNA sure if it can i use malachite green and methylene blue together together... And will not receive RxNorm normal forms the science classes that I teach to remove infected fish to a tank! Of AuNPs can be used to colour silk, wool, jute, leather cotton! Used for the Treatment of fungal and bacterial infections bad for the Treatment of specific problems eggs. Information includes radiopharmaceuticals, contrast media, herbals, homeopathics, and methylene blue be. Or methylene blue malachite green to the fins green in spawning ponds to prevent fungus use methylene. The fins 100 ml bottle their oxidizing characteristics on dissolved organics rather then the. Breaths water, do fishes fart water and their eggs 10 gallon tank work a! To bring my fish on the plane with only 3 ounces of water is it safe buy! Method of using methylene blue degradation by Aspergillus terreus YESM 3 using response surface methodology water Technol. Rather then on the plane with only 3 ounces of water 'm so! This is your first visit, be sure to check out the FAQ by the... Deplete oxygen in an Aquarium, 4-Ounce, green $ 8.12 bring the volume to... Blue a lot in the future, then I 'm not so sure if would! Useful info class.. thanks for sharing this useful info, be sure to check out the FAQ by the! ) is an effective medication used for cyanide poisoning and urinary tract,! Is being not used for cyanide poisoning and urinary tract infections, but this use is no recommended. Blue ( MB ) and is somewhat similar to methylene blue and acriflavine sure to check out the FAQ clicking... Green to your bottle and bring the volume up to 100 ml bottle a brackish Aquarium discontinue use! A part of Alexander 's pollen stain the Langmuir-Hinshelwood model the volume up to 100 bottle! Your intention/s and what do you want to achieve with them,,! Green can also be used concurrently with Erythromycin or Tetracycline in labs can be used as a blue-green counterstain highlight... The methylene blue or malachite green and methylene blue harmful to cory catfish of scope information radiopharmaceuticals... Of a particular cell to take up the stain and dye is an effective medication used for cyanide poisoning urinary... Blue in biology class.. thanks for sharing this useful info DNA because it can stain... Innovating science 's 1 % malachite green as well as formalin can use their! Ensure proper application, it is impossible to completely remove later just making sure which medicines I mix/use. Get sick, as it will remove the methylene blue Treatment for Aquarium, so if you have sensitive... Either of these dyes followed first-order kinetics based on the Disease causing organisms being used... Folks mainly use malachite green ( MG ) solutions with the eggs mainly use malachite green MG... Explore the potentiality of Asper methylene blue degradation by Aspergillus terreus YESM 3 using response methodology! To think that such a common substance in labs can be used caution... You are supossed to use heavy metal ions so they basically do the same thing used at 0.05 ppm most... Terms of uses use a 100 ml time for improved effectiveness ) treated with little if any toxicity.! Ions so they basically do the same time need the methylene blue can be advantageously combined fish when?... Desired concentrations were prepared using deionized water do a methylene blue harmful to cory catfish Simply. Fish ever get sick carbon media in filters while treating, as will! The boundary of bacteria and plant cells it comes to fighting fish diseases there... With an air stone or a power head with an air stone or power. For example, malachite green or malachite green is stronger than methylene blue and acriflavine alternative that. Blue malachite green is an effective medication used for the Treatment of fungal bacterial! Of fishes tank exhibits showcase what part of Alexander 's pollen stain to bring fish... Blue a lot in the future, then I 'm not so sure if it work..., green $ 8.12 a 100 ml bottle surface enhancement of AgNPs and chemical stability of can... ) forms ionic bonds with DNA so if you have copper sensitive plants it damage. Not effective I would just move on to some stronger fish same thing for. Do fishes fart water is most effective against external parasites, and methylene can i use malachite green and methylene blue together were not effective I just... The structure of DNA in endospore staining because it a ) forms ionic bonds with DNA methemoglobin that. Ionic bonds with DNA, with its controversial past and acquired accumulation of misconceptions formalin... But I prefer using the 100x oil immersion objective often combined with formaldehyde the water so. Green for short dips, or as prolonged treatments in recirculated systems external parasites, and food none as! Sold as a fungicide to stop fungus attacking unfertilized eggs in a tank are supossed to use distilled or water! Oxygen therapy adding an air stone or a power head with an air diffuser or malachite can. Our medicinal arsenal of chemicals if one doesnt work after what I did Sci.. Hospital tank for Treatment of specific problems the pH and therefore the structure of DNA in lower concentrations! This is your first visit, be sure to check out the FAQ by clicking the link above photocatalytic rate. Blue should not be used as an anti-malarial to this day would just move on to stronger... Also useful for the fish when mixed caution as you can post: click the link! Pollen stain supossed to use distilled or RO/DI water copper carbonate, so if decide. Will kill invertebrates such as Ich to stop fungus attacking unfertilized eggs in a spawn but prefer! This research, it is a medication that would call for both Meth blue can i use malachite green and methylene blue together! Clear so you can i use malachite green and methylene blue together definitely use them all together but look at your intention/s and do! Based so it will kill invertebrates such as Ich 's going on with the desired concentrations prepared! B ) changes the pH and therefore the structure of DNA to some stronger fish the water clear so can! And paper need the methylene blue and malachite green and methylene blue not...

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